Next Thursday, May 6th, at 3 pm, Natalia Ochocka from the Laboratory of Molecular Neurobiology led by Prof. Bozena Kaminska, will give a lecture entitled Single-cell RNA and protein sequencing reveals functional heterogeneity of glioma-associated brain macrophages
Microglia and bone marrow-derived macrophages accumulate and adapt a tumor-supporting role in human malignant gliomas that show prevalence in men. Brain resident microglia and peripheral macrophages have different origins and transcriptomic profiles that suggest distinct functions in tumorigenesis. However, the heterogeneity of the cell functional phenotype and specific roles in gliomas remain elusive. Here, we show scRNA-seq combined with surface protein labeling of CD11b+ cells sorted from experimental GL261 gliomas. The identified marker proteins showed distinct spatial distribution of identified subsets in glioma-bearing brains. We demonstrate that although microglia and monocytes/macrophages activate similar transcriptional networks within the tumor environment, the responses of monocytes/macrophages are stronger, and they are localized predominantly within the tumor core. Monocytes/macrophages show substantial transcriptional heterogeneity allowing to distinguish subsequent differentiation states. The differentiated macrophages upregulated immune-suppressive genes and exhibited a high level of PD-L1 protein, which points to their role in suppression of the immune response against the tumor. Additionally, we studied whether there are sex differences in the transcriptomes of myeloid cells infiltrating gliomas and found higher expression of MHCII encoding genes in glioma-activated male microglia. This finding was corroborated in bulk and scRNA-seq data from human diffuse gliomas. Our results suggest that sex-specific gene expression in glioma-activated microglia may be relevant to differences in incidence and outcomes of glioma patients.
Meeting ID: 966 1729 0469
31 maja 2021 r. o godz. 14:00 odbędzie się on-line publiczna obrona rozprawy doktorskiej na stopień doktora nauk ścisłych i przyrodniczych w dyscyplinie nauki biologiczne mgr. Kacpra Kondrakiewicza pt. „Characterization of central amygdala circuits activated by social transfer of fear”
Promotor: dr hab. Ewelina Knapska, prof. Instytutu Nenckiego PAN
Recenzenci: prof. dr hab. Jarosław Barski, prof. Christian Keysers, prof. Marta Moita
Link do obrony: https://zoom.us/j/95883512522?pwd=a0k3dlQxY1J4NTByWnNHeVlvNzlBdz09
Link do streszczenia i recenzji: https://cloud.nencki.gov.pl/d/db25b3c727/
Zoom Nencki is inviting you to a scheduled Zoom meeting.
Topic: Zaproszenie na obronę mgr. Kacpra Kondrakiewicza
Time: May 31, 2021 01:30 PM Warsaw
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Meeting ID: 958 8351 2522
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Meeting ID: 958 8351 2522
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Nencki Institute as a representative of SPARK Poland within the SPARK Europe is given an opportunity to be a host of the next webinar of European SPARKs joint series on May 5th, 16-17 CET.
We would like to cordially invite you to participate in the webinar titled “IP Strategies for Inventions in Biotech” during which we will get an overview of relevant IP rights for biotech innovations, the context of IP in product development, and characteristics of IP protection in life sciences. The speaker who will share with us his experience is Dr. Sebastian Tegethoff, an experienced legal adviser in developing IP strategies for research institutes and biotech companies, lecturer, and expert of European IP Helpdesk.
The series of webinars is open for all students and employees of SPARK associated organizations (Nencki Institute PAS and International Institute of Molecular and Cell Biology in Warsaw).
Registration to the webinar is required in advance. Please register at: https://zoom.us/meeting/register/tJwtdO-prDwrGNAteCs_0xWESpwtzPBL6lnh
latest on May 5th at 14 CET. With later registrations, access cannot be guaranteed. There are a limited number of places available and they are filled in in the order of registration.
As a reminder, webinars will take place biweekly on Wednesday at 16 -17 CET and will cover topics from design thinking to challenges in selling complex high-tech products from top-quality speakers around Europe and the US.
Serdecznie zapraszamy na kolejny wykład organizowany przez Fundację Nenckiego 12 maja o godz. 16.00. Dr Iwona Czaban z Laboratorium Biologii Molekularnej Nowotworów CENT UW przedstawi wykład p.t.: „Jak powstają nowotwory i dlaczego są tak niebezpieczne”
Wykład odbędzie się na platformie zoom pod linkiem: https://zoom.us/j/97697626284